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naïve macs sort kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec naïve macs sort kit
    Expected results of this protocol <t>Naïve</t> CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.
    Naïve Macs Sort Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/na%C3%AFve+macs+sort+kit/Naive+CD4%2B+T+Cell+Isolation+Kit%2C+mouse/pmc13129426-229-18-23
    Average 97 stars, based on 445 article reviews
    naïve macs sort kit - by Bioz Stars, 2026-11
    97/100 stars

    Images

    1) Product Images from "Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells"

    Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2026.104519

    Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.
    Figure Legend Snippet: Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.

    Techniques Used: Cell Culture, Dot Blot, Luminex

    Related Articles

    FACS:

    Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells
    Article Snippet: .. Note: If no FACS device is available, the sorting of naïve T cells can be performed using a naïve MACS Sort Kit from Miltenyi Biotec (130-104-453 ). ..

    Magnetic Cell Separation:

    Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells
    Article Snippet: .. Note: If no FACS device is available, the sorting of naïve T cells can be performed using a naïve MACS Sort Kit from Miltenyi Biotec (130-104-453 ). ..



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    Image Search Results


    Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.

    Journal: STAR Protocols

    Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells

    doi: 10.1016/j.xpro.2026.104519

    Figure Lengend Snippet: Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.

    Article Snippet: Note: If no FACS device is available, the sorting of naïve T cells can be performed using a naïve MACS Sort Kit from Miltenyi Biotec (130-104-453 ).

    Techniques: Cell Culture, Dot Blot, Luminex